Optimization for Keratinase Enzyme Production Using Bacillus thuringiensis TS2

Keratinase producing bacterial strains were isolated from the chicken feather dumping site. Keratinase producing bacterial strains were identified by physical, biochemical characteristics, fatty acid methyl ester (FAME) and 16S rDNA sequences. Medium optimization of the selected keratinase producing bacterial strain (Bacillus thuringiensis TS2) was checked with various parameters such as incubation time (52.3±1.2 U/mg), substrate concentration (41.3±1.2 U/mg), inoculums concentration (43.54±0.89 U/mg), carbon sources (76.20±0.34U/mg), nitrogensources (85.60±3.12U/mg), pH (90.78±0.97U/mg), temperature (92.78±0.59U/mg) and chemical inhibitors (112.17±0.02U/mg) that were tested with specific keratinase activity.